While the defective gene product in patients with PKU was identified using biochemical analysis, how was the cystic fibrosis gene identified?
What will be an ideal response?
The cystic fibrosis gene was identified by a combination of genetic and modern molecular biology techniques. It was first localized to chromosome 7, and then molecularly cloned and sequenced. The sequence of the mutant allele was compared with the allele of a normal person to discover a three base-pair deletion in the cystic fibrosis gene. To determine the gene's function, its amino acid sequence and three-dimensional structure was deduced. This was compared with a database of protein sequences to determine the gene's function as an active transport protein.
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Among the last line of defenses against prolonged exposure to an extracellular pathogen is _____
A) lysozyme production B) phagocytosis by neutrophils C) antibody production by plasma cells D) histamine release by basophils E) lysis by natural killer cells
Once an amino acid has been attached to the appropriate tRNA, the tRNA is called a(n) ________, and the amino acid is said to be ________
Fill in the blank with correct word.
Facilitated diffusion is a type of
What will be an ideal response?
Clusters of budding yeast and branching pseudohyphae are consistent with (candidiasis/dermatophytosis/histoplasmosis).
Fill in the blank(s) with the appropriate word(s).