Explain the difference between the two FISH Class I probes: alpha satellite and telomere

probes.

What will be an ideal response


Class I probes are generated against repeated sequences. Alpha satellites are monomers repeated tandemly around
the centromere of a chromosome while telomeres are repeated TTAGGG sequences at the ends of the chromosomes.
Alpha satellites are mostly identical, but there is sufficient polymorphism to identify most chromosomes uniquely
using alpha satellite probes. Telomere repeats vary between individuals and decrease with age, so telomere probes
cannot accurately identify chromosomes, but they can be used to locate and quantify all telomere sequences.

Health & Biomechanics

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