Michelle wants to know which cells in a particular organ produce a specific gene product. She has a cDNA clone of the gene available. What method would be the most useful to answer this question?
A) Sanger sequencing of the clone
B) real time PCR
C) FISH (fluorescent in situ hybridization)
D) restriction fragment analysis
E) microinjection of the gene fragment
C
Bloom's Taxonomy: Analysis
Section: Applications of Recombinant DNA Technology
Learning Outcome: 8.17
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Production of the iron-storing protein ferritin is regulated by aconitase, which binds to a 30-nucleotide sequence at the beginning of the ferritin mRNA and interferes with ribosome binding. Aconitase must be a
A. transcription repressor protein. B. translation repressor protein. C. RNA interference protein. D. translation initiation protein. Clarify Question · What is the key concept addressed by the question? · What type of thinking is required? · What key words does the question contain and what do they mean? Gather Content · What do you already know about translational regulation? Consider Possibilities · Consider the different answer options. Which can you rule out? Choose Answer · Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer? Reflect on Process · Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
A distinctive type of larva shared by several phyla of invertebrates and considered to be important in phylogeny is the
A. trochophore. B. paedomorph. C. gastrula. D. metamere. E. tadpole.
Marijuana is considered a(n)
a. stimulant. b. depressant. c. hallucinogen d. analgesic e. neurotransmitter.
The sequence of the coding strand of a DNA molecule (that is, the DNA strand that contains the codons specifying the protein sequence) is 5'-CGGATGCTTA-3'. What is the sequence of the RNA made from this DNA?
a. 5'-UAAGCAUCCG-3' b. 5'-CGGAUGCUUA-3' c. 5'-AUUCGUAGGC-3' d. 5'-GCCUACGAAU-3' e. 5'-TAAGCATCCG-3'