Explain why GC-rich DNA requires a higher temperature to denture or melt than AT-rich DNA and hypothesize as to why the GC content of chromosomes in microorganisms from different environments varies widely
What will be an ideal response?
Answer: The overall increase in stability of G-C pairing should be emphasized over A-T binding. A DNA strand with high GC content (low AT) has more triple H bonds compared to an AT rich (low GC) strand of the same length contains mostly double H bonds. More energy is therefore required to break more H bonds, and thus GC rich DNA is more heat resistant to denaturation. The fact that GC rich DNA is more heat resistance may mean that microorganisms adapted to high temperature environments may have chromosomes with higher GC content than microorganism in moderate or low temperature environments.
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Fill in the blank(s) with correct word
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Fill in the blank(s) with the appropriate word(s).