A tube coagulase test was performed on catalase-positive, gram-positive cocci that were ?-hemolytic and grew on mannitol salt agar. The tube was checked after overnight incubation and was negative. Which of the following statements explains this discrepancy?

a. A tube coagulase should not be performed on a catalase-positive organism.
b. A ?-hemolytic colony, which is catalase positive and grows on mannitol salt, is normally coagulase negative.
c. The test should have been read in 1 to 4 hours in case any hemolysins are present.
d. No discrepancy is present; the results are accurate.


C
Some strains of S. aureus produce fibrinolysin, which will dissolve the clot after 4 hours of in-cubation at 35°C and may appear to be negative if allowed to incubate longer than 4 hours.

Biology & Microbiology

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