Imagine that you are using random PCR primers to amplify fragments of DNA. Would you expect to get more amplification products if you set the PCR machine at a higher or lower annealing temperature and why?
What will be an ideal response?
Higher annealing temperatures increase "stringency," meaning the accuracy of base complementarity must be higher for successful annealing. Under such conditions, fewer successful amplifications are expected compared to conditions in which stringency has been lowered by using a lower annealing temperature.
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The structure used to amplify an elephant seal bull's roar is a(n):
a. inflated proboscis. b. hallow tusk used as a horn. c. sirenaphores. d. blowhole. e. melon.
Microscopic algae typically fall under the classification of:
a. nekton. b. phytoplankton. c. zooplankton. d. benthos. e. profundal microorganisms.
Guttation results from:a
water pressure. b. transpiration pull. c. osmotic pressure. d. root pressure. e. sink-to-source transport
Proteins are made at the
A. nucleolus. B. ribosome. C. Golgi apparatus. D. grana.