In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and ligate the gene into a plasmid vector. However, even though all of the controls work, no transcription is obtained using your fragment. Working backward, you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene. It is possible that during restriction

digestion

A) the termination signal was removed.
B) the RNA polymerase became inactivated.
C) primase could not bind.
D) the promoter was removed
E) none of the above


D) the promoter was removed

Biology & Microbiology

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An example of an involuntary response would be:

A. secretion of saliva. B. sighing. C. heart rate. D. both secretion of saliva and heart rate.

Biology & Microbiology

Lipids are polar, hydrophilic molecules

Indicate whether this statement is true or false.

Biology & Microbiology

What is the primary role of bile salts during

digestion?

a. osmoregulation b. to kill bacteria that may have been consumed c. to coat fat droplets and keep them separated into small droplets d. to enzymatically digest fats e. to do all of these

Biology & Microbiology

A housefly is an example of a(n) ______ vector

a. mechanical b. line c. biological

Biology & Microbiology