You are a researcher interested in studying the cytoskeleton. How can you use antibodies to locate actin- and tubulin-containing structures?
What will be an ideal response?
Since those cytoskeletal components are proteins, it is possible to immunize an animal with foreign proteins isolated from tissues. For example, a rabbit could be immunized with the actin and tubulin purified from tissues of the chicken, a very distant relative of the rabbit. Those antibodies could be purified from the rabbit's blood and then linked to fluorescent tags. When the cells are exposed to the anti-actin and anti-tubulin antibodies, they will stick to those molecules in the cell. If different colored dyes were used to tag the antibodies, it would be possible to localize those cytoskeletal proteins in the same cell.
You might also like to view...
Which of the following is TRUE about the concentration gradient of Na+ in a neuron at rest?
A. It is in the same direction as the diffusion potential due to the concentration gradient for K+. B. It favors movement of Na+ in the opposite direction as the electrical potential acting on Na+ at the resting membrane potential. C. It favors its movement into the cell at the resting membrane potential. D. It favors its movement out of the cell at the resting membrane potential. E. It is equal and opposite to the electrical potential acting on Na+ at the resting membrane potential.
Which vessels collect deoxygenated blood from the myocardium?
A) coronary arteries B) cardiac veins C) atrial arteries D) marginal arteries
Which functional group of muscles tends to oppose and slow the action of prime movers?
A. Fixators B. Agonists C. Synergists D. Antagonists
The olecranon process is found on the
A. ulna. B. radius. C. scapula. D. humerus. E. clavicle.