The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is
A) isolate the desired gene — purify the expressed protein — grow the transformed cells into suitable quantities — insert the gene into an expression vector — transform host cells with the expression vector.
B) purify the expressed protein — insert the gene into an expression vector — transform host cells with the expression vector — grow the transformed cells into suitable quantities — isolate the desired gene.
C) isolate the desired gene — insert the gene into an expression vector — transform host cells with the expression vector — grow the transformed cells into suitable quantities — purify the expressed protein.
D) transform host cells with the expression vector — grow the transformed cells into suitable quantities — isolate the desired gene — insert the gene into an expression vector — purify the expressed protein.
E) grow the transformed cells into suitable quantities — transform host cells with the expression vector — insert the gene into an expression vector —isolate the desired gene — purify the expressed protein.
Answer: C
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