You are interpreting data on a DNA chip or microarray. You expose the chip to a mixture of two cDNA populations: one from cells that were not treated with a glucocorticoid hormone (untreated controls; labeled with a red fluorescent dye) and one from cells that were treated with glucocorticoid hormones (glucocorticoid-treated; labeled with green fluorescent dye). You look at a spot on the chip representing the gene for glutamyltransferase and it is green. How do you interpret this result?
a) The glutamyltransferase gene is expressed in the control cDNA population, but not in the glucocorticoid-treated population.
b) The glutamyltransferase gene is expressed essentially equally in both cDNA populations.
c) The glutamyltransferase gene is not expressed in the control cDNA population, but it is expressed in the glucocorticoid-treated population.
d) The glutamyltransferase gene is not affected by glucocorticoid treatment.
Ans: c) The glutamyltransferase gene is not expressed in the control cDNA population, but it is expressed in the glucocorticoid-treated population.
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