What is the primary stain, mordant, decolorizing agent, and counterstain of the Gram stain protocol? What is the purpose of each? How does the protocol result in gram-positive cells staining purple and gram-negative cells staining pink?

What will be an ideal response?


Ans: Primary stain: imparts color on all cells
Mordant: to intensify the color of the primary stain
Decolorizing agent: may or may not remove the primary stain from the cell
Counterstain: if the primary stain is removed, the decolorized cellular components will accept and assume the contrasting color of the counterstain.

Biology & Microbiology

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