Compare and contrast the two major categories of restriction enzymes
What will be an ideal response?
All restriction enzymes recognize specific sequences in DNA molecules and cut at those sequences, making them powerful tools for generating and manipulating DNA fragments. However, these enzymes differ in the type of cut they make. One type of cut is a staggered cut, which produces complementary overhangs known as "sticky ends." These ends can base pair with each other (if they have the same sequence), and they allow DNA fragments to be joined relatively easily. The other type of cut occurs at the same place on each strand of DNA and produces "blunt ends." Fragments with this type of end do not base pair with each other at all, making the joining of these fragments more difficult than sticky-ended fragments. However, blunt ends are more "generic" than sticky ends; they allow two fragments to be joined no matter which restriction enzyme generated them (as long as they were generated by blunt-end cutters).
Bloom's Taxonomy: Analysis
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9
You might also like to view...
The cardiac, pyloric, anal, and ileocecal sphincters are all part of the
(a) digestive tract. (b) respiratory tract. (c) circulatory system. (d) muscular system.
Fruit often has fleshy tissues that develop from the:
a. endosperm b. ovule c. seed coat d. cotyledon e. ovary wall
Adhesion proteins and ____ help guide the growth of the pollen tube in the proper direction towards the egg
a. differentiation upon contact with the flower b. exposure to moisture c. cell signaling pathways d. electromagnetic impulses from the flower e. insertion of the pollen tube
Old pine stumps are dug up, split into pieces and sold as "fatwood." Fatwood is used as a fire starter. Why is this done?
A. Pine bark does not absorb water and is flammable. B. The high concentration of volatile organic compounds makes the wood flammable. C. The wood in the stumps is porous and dry, thus it burns easily.