It is not necessary to use a male from a tester strain to genetically map genes on the X chromosome. How can this be done and why does it work?

What will be an ideal response?


It is necessary to start with females that are heterozygous for X-linked genes. They can be bred with any male. Their male offspring can then be used to determine linkage distances. Male offspring will have only a single X chromosome, and that chromosome will come from the mother, so they can be used to measure recombination frequencies to construct a genetic map.

Biology & Microbiology

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