How and why is the two-hybrid system used to find where two proteins interact? Use a real example
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Two-hybrid screening is used to detect protein-protein or protein-DNA interactions by testing for physical binding. You will look for the activation of a reporter gene when a transcription factor binds to an upstream activating sequence. In a two-hybrid screen, the transcription factor is broken into two fragments: the binding domain (BD), which binds to the UAS, and the activation domain (AD), which activates transcription of the reporter gene. For instance, the yeast two-hybrid screen uses a protein that is translationally fused to a Gal4 BD and another protein to a Gal4 AD.
If the proteins interact, the two Gal4 elements are brought together and transcription occurs.
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What will be an ideal response?
The portion of the body fluid contained in cells is
a. interstitial fluid. b. plasma. c. intracellular fluid. d. extracellular fluid.
Organisms in the genus Pseudomonas are resistant to quats.
Answer the following statement true (T) or false (F)
The term herbaceous implies that a plant
A. is only found in wet environments. B. probably will live several years. C. has no apical meristem. D. has not developed a secondary vascular tissue. E. lacks vascular tissue.