Describe the Southern blotting procedure for analyzing DNA
What will be an ideal response
A piece of DNA to be analyzed is digested by restriction enzymes. The resulting fragments are separated by
agarose electrophoresis. The gel is soaked in an alkaline solution to convert the DNA to single-stranded form. Then
a nitrocellulose membrane is laid over the agarose. The DNA is transferred to the nitrocellulose by "blotting." The
blotting traditionally was done by capillary action, but newer techniques employ either a vacuum or an electrical
field to speed the process. Since the DNA is now single-stranded and fixed to the nitrocellulose, specific sequences
can easily be detected using nucleic acid probes.
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Fill in the crossword puzzle with the words missing from the sentences below. Match the number of the sentence to the boxes placed across or down the grid. If filled out correctly, the words will fit neatly into the puzzle.
 ACROSS 2 A general term for a disease affecting the nail is: ________. 7 The ________ system forms the entire surface area of the body. 8 ________ is a superficial form of dermatitis, with pruritus and scaliness of the epidermis as the primary symptoms. 9 Actinic ________ is a precancerous condition of the skin caused by exposure to sunlight. DOWN 1 The clinical term for “pimple” is: ________. 3 A common injury to the skin caused by scraping produces a superficial wound called a(n) ________. 4 ________ is an abnormally pale color of the skin. 5 If an abscess is associated with a hair follicle, the local swelling on the skin is called a(n) ________. 6 A(n) ________ is a minor surgery involving the removal of tissue for evaluation. ?
What is 12% of 120?
A) 144 B) 14.4 C) 1.44 D) 0.144 E) 1440
Which procedure is the surgical fusion of two bones to stiffen a joint?
A) arthroscopy B) internal fixation C) arthrolysis D) arthrodesis
The health consequences of carrying excess weight can include increased incidence of Type 2 diabetes, lower back pain, and adhesive capsulitis of the shoulder
Indicate whether the statement is true or false