You are attempting to clone your first gene. Up to the point of screening, everything has gone well. The vector has a gene for ampicillin resistance. In addition to the gene DNA, the insert also carries a gene for streptomycin resistance. Based on restriction digest, if properly inserted into the vector, your inserted DNA should be located within the ampicillin gene. Therefore, when screening,

you should look for clones that are

A) ampicillin resistant and streptomycin resistant.
B) ampicillin sensitive and streptomycin sensitive.
C) ampicillin resistant and streptomycin sensitive.
D) ampicillin sensitive and streptomycin resistant.
E) Not enough information is given to determine the answer.


D) ampicillin sensitive and streptomycin resistant.

Biology & Microbiology

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