) How can NanoSIMS be used to determine which Roseobacters most strongly use DMSP as a sulfur source within a coastal marine environment? Explain the NanoSIMS methodology in your answer

What will be an ideal response?


Answer: NanoSIMS is a highly sensitive technique that can be combined with other methods to quantify the relative proportions of isotopic atoms such as 34S incorporated into various isolated organisms from 34S~DMSP exposure. The organisms containing the highest 34S are likely those that assimilate DMSP the most. In NanoSIMS, a high-energy ion beam is directed onto a sample to break apart most chemical bonds such that secondary ions released represent the composition of the sample. The various secondary ions are quantified with a mass spectrometer.

Biology & Microbiology

You might also like to view...

When reporting a direct smear from a primary specimen, it is important to note the presence and quantity of white blood cells even in the absence of bacteria for all of the following reasons ex-cept:

a. the pathogen may not be present in suffi-cient quantities. b. the presence of white blood cells indicates a purulent process. c. the pathogen may not be present in the sample collected. d. it is not important to report white blood cells unless bacteria are present.

Biology & Microbiology

The level of the oceans is rising due to ____

a. ozone depletion b. global warming c. lost species biodiversity d. increased precipitation e. acid rain

Biology & Microbiology

What feature of a dinosaur determines whether it is a saurischian or an ornithischian dinosaur?

a. size b. habitat c. feeding preference d. pelvic bone structure e. number of feet it walks on

Biology & Microbiology

Which of the following provides information about the nature and potential cause of a fungal infection the most quickly?

A. Culture on Sabouraud's dextrose agar B. Testing for temperature dimorphism C. Culture on selective and differential media D. Direct examination of fresh specimens (wet mounts, KOH preps, brighteners)

Biology & Microbiology