Define upstream and downstream processing when producing a protein
What will be an ideal response?
Upstream processing is the actual cell line expression of the protein of interest.
Downstream is the purification of the protein from all the other components in the cell to ensure
purity.
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H1 binding to DNA is different than that of the other four histones because H1 binds to:
A. the major groove of the DNA only. B the DNA as it comes off both sides of the nucleosome. C. as a component of the octamer; however, unlike H2A, H3, and H4, H1 lacks the histone tail. D. one strand of the linker DNA as it comes off the nucleosome and binds a second site in the central region of the DNA supercoil. E. the DNA as a homotetramer.
The total compaction of DNA as seen in metaphase chromosomes is approximately ________-fold.
A) 10,000 B) 50,000 C) 100,000 D) 40
Mouse eye genes can be inserted into Drosophila and can regulate the flies' eye formation. Differences in size and shape of a zebra are controlled by a gene also found in zebrafish. What do these examples illustrate?
What will be an ideal response?
Southern blotting is a process where __________ is transferred from a gel to a __________ where a
probe can be applied. a. DNA; water solution b. protein; water solution c. DNA; nitrocellulose filter d. RNA; nitrocellulose filter e. protein; nitrocellulose filter