In reverse genetics, what is the correct order in which the experimenter proceeds?
A) silencing the genes in question using RNAi, followed by generating gain-of-function alleles
B) screening genes via PCR, followed by deletion of the flanking sequences
C) selection of a phenotypic alternative for the gene in question and sequencing its DNA
D) induction of and selection for a mutant allele with a known sequence, followed by screening for mutations of interest
E) random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits
D
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In your observations of a new rotifer species, you note that diploid females can lay diploid eggs that in turn become diploid female adults. What do you conclude about this behavior?
A. This is asexual reproduction by way of parthenogenesis. B. This asexual reproduction by way of budding of a new individual from the parent. C. This is sexual reproduction by way of parthenogenesis. D. This is sexual reproduction because sperm must have been stored in the female for later fertilization of her eggs. E. This is sexual reproduction by way of diploid females laying haploid eggs that hatch into haploid males who in turn produce sperm that fertilizes other haploid eggs.
A fertilized moth egg passes through which stages of
development before becoming an adult? I. larval II. pupal III. reproductive a. I only b. II only c. I and II d. I and III e. II and III
Which of the following processes is not involved in the hydrologic cycle?
a. chemosynthesis b. transpiration c. precipitation d. evaporation e. condensation
When bound to the DNA, the repressor protein usually prevents attachment of the RNA polymerase to the ________.
A. promoter B. operon C. regulator D. operator