To study the promoter of a gene, a researcher should use a cDNA library.
Answer the following statement true (T) or false (F)
False
Clarify Question
What is the key concept addressed by the question?
This question is asking you what type of library is best to study the promoter of a gene.
What type of thinking is required?
You are being asked to take what you already know about DNA libraries and use, or apply, it to a specific experimental question.
What key words does the question contain and what do they mean?
Promoter, a sequence of DNA that controls when and where transcription will begin
cDNA library, a collection of recombinant vectors that have cDNA inserts
Gather Content
What do you already know about cDNA? How does it relate to the question?
Recall that cDNA is made from mRNA as a starting material using reverse transcriptase. A cDNA library contains the coding sequences of genes.
Consider Possibilities
What other information is related to the question? Which information is most useful?
By contrast, in a genomic DNA library, inserts are derived from chromosomal DNA (not just the coding regions).
Choose Answer
Given what you now know, what information and/or problem-solving approach is most likely to produce the correct answer?
In this question, you are interested in the promoter of a gene. Recall that a promoter is generally found upstream of a gene, and is not considered to be part of the coding region of a gene. Therefore, a cDNA library would not be useful to study a gene's promoter, and the answer to the question is "False".
Reflect on Process
Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
This question asked you to consider the best way to study the promoter of a gene. Answering this question correctly depended on your ability to use the concept of different types of DNA libraries in an experimental situation. If you got the correct answer, great job! If you got an incorrect answer, where did the process break down? Did you remember the difference between library types? Did you recall that a promoter is not part of the coding region of the DNA?
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