You are studying the function of a recently identified gene in C. elegans. You perform genetic screens for several months in an attempt to isolate loss-of-function gene mutations, but your efforts are unsuccessful. Your advisor suggests you try another approach to eliminate gene function. The best technique to accomplish this goal would be

A. use a C. elegans strain with a homozygous TFIID mutation to prevent the translation initiation complex from forming.
B. use RNA interference to prevent mRNA translation.
C. to design a repressor to bind to the operon of this gene.
D. use a histone deacetylase to induce a transcriptionally inactive state.


Answer: B

Biology & Microbiology

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