A plasmid cloning vector and DNA fragments containing the XYZ gene, each digested with the same restriction
enzyme, are mixed with DNA ligase. Which product(s) will result?
a. recombinant plasmids only
b. nonrecombinant plasmids only
c. joined-together copies of the DNA fragment containing the XYZ gene only
d. a mixture of recombinant plasmids, nonrecombinant plasmids, and joined-together copies of the DNA
fragment containing the XYZ gene
e. no reaction will occur because DNA polymerase rather than DNA ligase is needed to insert the DNA
fragments into the plasmid cloning vector
ANSWER: d
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Fill in the blank(s) with the appropriate word(s).
Mutations in proto-oncogenes create
What will be an ideal response?