The determination of carbohydrate utilization patterns for the differentiation of Neisseria spp. and M. catarrhalis is best performed by:
a. incubation of inoculated cysteine trypti-case soy agar (CTA) with 1% dextrose, maltose, lactose, and sucrose for 48 to 72 hours.
b. oxidative-fermentative (OF) medium with dextrose, maltose, lactose, and sucrose.
c. heavy inoculation of buffered, low-peptone substrate with dextrose, maltose, lactose, and sucrose.
d. carbohydrate testing strips.
C
The determination of carbohydrate utilization patterns has historically been performed in CTA with 1% dextrose, maltose, lactose, and sucrose. This medium is no longer widely used; it does not work well for oxidative Neisseria spp., specifically N. gonorrhoeae and N. meningitidis. Therefore carbohydrate utilization patterns are currently determined by inoculating an extremely heavy suspension of the organism to be tested in a small volume of buffered, low-peptone sub-strate with the appropriate carbohydrate.
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