A researcher identified an uncharacterized mouse gene and the protein it encoded. The researcher prepared antibodies to the purified protein. To determine the specificity of the antibody the researcher incubated tissue sections from a knock-out mouse with the new antibody. If the antibody were specific for the protein under study, would you expect to see labeling in these tissue sections?

a. Yes, because antibody binding does not involve gene products.
b. Yes, because the protein is a mouse protein.
c. Yes, because the gene was knocked out, not the protein.
d. No, because the protein cannot be expressed if the gene is absent.
e. No, because proteins are too small to visualize under a light microscope.


D

Anatomy & Physiology

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