A dark pigmented colony is isolated from the leg wound of a fisherman from Florida. The gram-negative rod grows on sheep blood and MacConkey agars. The organism provides the following reactions:
TSI: K/A Indole: no color change Lysine: no color change
1% NaCL: no growth Arginine: purple Ornithine: no color change
DNase: pink halo around the area of organism inoculum
The microbiologist cannot read the oxidase reaction due to the dark pigment. What can he do to help achieve a valid oxidase reaction?
A) Grow the organism under anaerobic conditions and retest
B) Grow the organism on a non-nutrient agar and retest
C) The oxidase test is not necessary for the identification of this organism
D) Perform a modified oxidase test
A
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