The following are steps in using SSU rRNA molecules to develop phylogenetic trees. Place these steps in the correct order.
_____
Count the number of nucleotide differences between each pair of sequences and calculate the evolutionary distance._____
Input data into computer and use appropriate software to construct a phylogenetic tree._____
Align nucleotide sequences to compare._____
Isolate DNA from cells of each organism being tested._____
Amplify the DNA of the SSU rRNA genes of each organism using polymerase chain reaction._____
Determine the nucleotide sequence of the SSU rRNA genes of each organism.

Fill in the blank(s) with the appropriate word(s).


5, 6, 4, 1, 2, 3

Biology & Microbiology

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