List the steps in a complement dependent cytotoxicity assay used for HLA antigen

typing.

What will be an ideal response


A complement dependent cytotoxicity assay proceeds as follows:
1. Aliquots of cells are mixed with different HLA antibodies and incubated.
2. Complement is added.
3. Supravital dye is added. Injured cells cannot exclude ethidium bromide and will be orange while intact cells are
capable of retaining carbonylmethyl fluorescein diacetate and appear green.
4. Formalin is added to fix cells.
5. Lysis is determined by the presence of dye in cells.

Health & Biomechanics

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Which are the largest of the paranasal sinuses??

A) ?maxillary sinuses B) ?frontal sinuses C) ?ethmoid sinuses D) ?sphenoid sinuses

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One of the few lasting gifts a physical educator can give children is to teach them about

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