Describe the three main steps to clone a gene into an organism

What will be an ideal response?


Answer: First, the DNA sequence of interest (gene) must be isolated. The isolated DNA is often larger than the gene of interest, so cutting the short gene sequence out of the large sequence is often performed, which uses restriction enzymes. Inserting the gene into a vector is the second main step. This usually requires DNA ligase whether the insert and vector are blunt- or sticky-ended. The final step involves transforming an (host) organism to house the clone vector.

Biology & Microbiology

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All of the following are fungi except:

A) molds. B) Paramecium. C) Penicillium. D) yeasts.

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Which structure prevents food from traveling down the trachea?

A) Pharynx B) Bronchus C) Glottis D) Epiglottis

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In 1996, a cloning experiment produced the sheep named Dolly. Contrary to the more traditional method of cloning by embryo splitting, Dolly was produced by which procedure?

What will be an ideal response?

Biology & Microbiology

Which of the following is an example of a neural function?

a. secretion of products b. contraction when stimulated c. protection from invasion by bacteria d. facilitation of diffusion

Biology & Microbiology