DNA sequencing using the dideoxy sequencing method involves PCR reactions using Taq polymerase, which we learned in this chapter can introduce errors because it lacks a proofreading mechanism

What precaution(s) can be taken in genomic or other sequencing projects to minimize the possibility of producing DNA sequences with errors?


The most common precaution taken is sequencing a fragment of DNA in both directions. Any replication errors will show up as mismatches, which could be resolved by resequencing that fragment of DNA.

Biology & Microbiology

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Which of the following statements regarding vaccines as disease preventatives is incorrect?

A. There is currently no smallpox vaccine available to the general public. B. Immunization is currently not possible against Clostridium perfringens. C. Zostavax is recommended for older adults to prevent shingles. D. The Carter Center has promoted the development of a vaccine against river blindness.

Biology & Microbiology

A group that contains species from separate evolutionary lineages is considered ____

a. paraphyletic b. polyphyletic c. monophyletic d. haplophyletic e. multiphyletic

Biology & Microbiology

In a population of 50,000 diploid individuals, what is the probability that a new neutral mutation will ultimately be lost from the population?

a. 0.99 b. 0.999 c. 0.9999 d. 0.99999 e. 0.999999

Biology & Microbiology

The plant pathogen ________ is a member of the alphaproteobacteria that alters plant cells by introducing DNA into them

A) Azotobacter B) Agrobacterium C) Rhizobium D) Pseudomonas E) Bdellovibrio

Biology & Microbiology