A 26-year-old woman who has a Gardnerella vaginalis infection becomes immunocompromised, which causes the organism to enter the bloodstream. She has several fever spikes; therefore blood cultures are drawn and placed in blood culture media containing sodium polyanethol sulfonate (SPS). After 5 days of incubation, the blood cultures are negative. Which of the following statements explains this

discrepancy?

a. G. vaginalis stays localized in the female genital tract and does not enter the blood-stream.
b. The blood culture media is most likely expired.
c. The automated blood culture system is most likely not correctly functioning.
d. G. vaginalis is usually inhibited by this anticoagulant, making recovery difficult.


D
In addition to its anticoagulant properties, SPS is also anticomplementary and antiphagocytic and interferes with the activity of some antimicrobial agents, notably aminoglycosides. SPS, however, may inhibit the growth of a few microorganisms, such as some strains of Neisseria spp., G. va-ginalis, Streptobacillus moniliformis, and all strains of Peptostreptococcus anaerobius.

Biology & Microbiology

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