Compare and contrast the two major categories of restriction enzymes.

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All restriction enzymes recognize specific sequences in DNA molecules and cut at those sequences, making them powerful tools for generating and manipulating DNA fragments. However, these enzymes differ in the type of cut they make. One type of cut is a staggered cut, which produces complementary overhangs known as "sticky ends." These ends can base pair with each other (if they have the same sequence), and they allow DNA fragments to be joined relatively easily. The other type of cut occurs at the same place on each strand of DNA and produces "blunt ends." Fragments with this type of end do not base pair with each other at all, making the joining of these fragments more difficult than sticky-ended fragments. However, blunt ends are more "generic" than sticky ends; they allow two fragments to be joined no matter which restriction enzyme generated them (as long as they were generated by blunt-end cutters).

Biology & Microbiology

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Which of the following areas is not known to contain stem cells?

A) the skin B) bone marrow C) the umbilical cord D) muscle

Biology & Microbiology

If you were cast up on a desert island with no

fresh water to drink, the level of which of the following would rise in your bloodstream in an effort to conserve water?

a. erythropoietin b. oxytocin c. insulin d. antidiuretic hormone e. glucose

Biology & Microbiology

Recombination analysis allows for the mapping of genes because the frequency of crossing over is proportional to what distance?

What will be an ideal response?

Biology & Microbiology

High-density conditions caused no noticeable change in the behavior of experimental wood frog (Rana sylvatica) tadpoles

Indicate whether the statement is true or false

Biology & Microbiology