Which of the following is the third step of a ChIP-Seq protocol?
A. digestion of chromatin with nuclease
B. washing away of unbound DNA, reversal of crosslinks, and removal of free histones
C. identification of DNA by sequencing
D. immunoprecipitation of nucleosome-DNA complexes with histone antibody
E. crosslinking of nucleosome histones to DNA in chromatin
D
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A silent mutation is a mutation that only occurs in noncoding regions of DNA
A. true B. false
Which cell type ranges in size from about 10 - 100 µm, has 80s ribosomes, and has internal compartments bounded by membranes?
A. Bacteria B. Eukarya C. Archaea
In ____________, fats accumulate on the inner walls of the arteries, narrowing the diameters of arteries and also leading to elevated blood pressure.
A. a heart murmur B. Purkinje fibrosis C. erythrocytosis D. atherosclerosis
Which statement about microarrays is false?
A) Microarrays enable scientists to determine the activity of thousands of genes at once. B) Microarrays use tiny portions of double-stranded RNA fragments from a large number of genes. C) Microarrays are used to determine which genes are active in different tissues or in tissues of different states of health. D) Microarrays use fluorescently labeled cDNA molecules to identify particular genes expressed at a particular time.