The control section containing both gram positive and gram negative organisms shows only gram positive organisms when stained with the Brown and Hopps technique. This is most likely the result of:

a) insufficient time in the Gallego solution
b) improper application of the crystal violet
c) insufficient time in the iodine solution
d) overdifferentiation with the picric acid-acetone


Answer: d) overdifferentiation with the picric acid-acetone

Biology & Microbiology

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