When studying carbohydrate metabolism in a newly identified bacterial species, you find the bacteria grow exclusively on the carbohydrate sucrose
a) Hypothesize whether the bacteria use glycolysis or a novel pathway for harnessing energy.
b) For what enzyme would you assay the bacteria to test your hypothesis?
What will be an ideal response?
Answer:
a) A reasonable hypothesis is that the bacteria use glycolysis, because sucrose can be cleaved by the enzyme sucrase into glucose and fructose, both of which can directly enter the glycolytic pathway as glucose-6-phosphate and fructose-6-phosphate, respectively.
b) Finding the presence of a sucrase enzyme would support your hypothesis.
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