The most common mutant allele of the PAH gene, which is responsible for the metabolic disorder PKU, has a SNP in the splice donor site of one intron. What is the simplest way to detect this allele?
A) PCR using genomic DNA as template and two primers, one on either side of the SNP, followed by gel electrophoresis
B) PCR using genomic DNA as template and two primers, one complementary to the region containing the SNP, followed by gel electrophoresis
C) PCR using two primers, one on either side of the SNP, followed by sequencing the PCR products
D) Direct exome sequencing using a microarray
E) Whole-genome sequencing
C) PCR using two primers, one on either side of the SNP, followed by sequencing the PCR products
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hormones?
a. they are active in an adult plant body b. they are signaling molecules c. they produce effects only at high concentrations d. they are produced only in certain regions of the plant e. all of these are NOT true of plant hormones
What is the role of primers in the PCR techniques?
A) To separate strands of double-stranded DNA B) To add free nucleotides to the newly formed DNA molecule C) To allow single strands of DNA to bind together D) To target specific areas of DNA
In the accompanying figure, the structure labeled as 4 is the:a
basilar membrane. b. tectorial membrane. c. organ of Corti. d. sterociliary membrane. e. vestibular membrane.
The ureters drain urine into the
(a) renal pelvis. (b) cecum. (c) urinary bladder. (d) small intestine.