How can the recircularization of a restriction-digested vector during a ligation reaction be minimized?

What will be an ideal response?


The restriction-digested vector can be treated with alkaline phosphatase to remove the 5' phosphates without which the DNA ligase is unable to form a phosphodiester bond.

Biology & Microbiology

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One technique for staining bacteria for viewing under the microscope is called the Gram stain. In this technique, alcohol is used as a decolorizer because it degrades the outer membrane found in some bacteria. What chemical component of the cell does alcohol affect?

A. Nucleic acids B. Carbohydrate C. Lipid D. Protein

Biology & Microbiology

What is the role of the endodermis in nutrient absorption? What will be an ideal response?

Biology & Microbiology

A microarray of thousands of DNA sequences stamped onto a glass plate is a ____

a. Polysome b. DNA chip c. Plasmid d. Polygene e. DNA plate

Biology & Microbiology

DNA replication

A) requires a type of RNA polymerase. B) is partially regulated by promoter/terminator sites. C) proceeds by making two continuous strands. D) is not edited once polymerization has occurred. E) is conservative.

Biology & Microbiology