How can the recircularization of a restriction-digested vector during a ligation reaction be minimized?
What will be an ideal response?
The restriction-digested vector can be treated with alkaline phosphatase to remove the 5' phosphates without which the DNA ligase is unable to form a phosphodiester bond.
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One technique for staining bacteria for viewing under the microscope is called the Gram stain. In this technique, alcohol is used as a decolorizer because it degrades the outer membrane found in some bacteria. What chemical component of the cell does alcohol affect?
A. Nucleic acids B. Carbohydrate C. Lipid D. Protein
What is the role of the endodermis in nutrient absorption? What will be an ideal response?
A microarray of thousands of DNA sequences stamped onto a glass plate is a ____
a. Polysome b. DNA chip c. Plasmid d. Polygene e. DNA plate
DNA replication
A) requires a type of RNA polymerase. B) is partially regulated by promoter/terminator sites. C) proceeds by making two continuous strands. D) is not edited once polymerization has occurred. E) is conservative.