Which of the following statements is true with respect to this in vitro replication system?
(a) There will be only one leading strand and one lagging strand produced using this template.
(b) The leading and lagging strands compose one half of each newly synthesized DNA strand.
(c) The DNA replication machinery can assemble at multiple places on this plasmid.
(d) One daughter DNA molecule will be slightly shorter than the other.
You decide to use different bacterial strains (each having one protein of the replication machinery mutated) in order to examine the role of individual proteins in the normal process of DNA replication.
(b) Leading and lagging strands are synthesized bidirectionally from the replication origin, and are joined togetherby DNA ligase where the two replication forks meet at the termination site. Choice (a) is not correct, because this answer implies that the replication fork is not bidirectional and that replication continues around the plasmid until the process makes it back to the origin of replication. Choice (c) is incorrect because the origin is a specialized sequence where initiator proteins bind and open the DNA so that the DNA replication machinery can assemble. Choice (d) is incorrect because the daughter DNA molecules will be same size as the original plasmid (and each other).
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