The explosive chemical trinitrotoluene (TNT) can remain in soils after use and is hazardous to humans. Propose an experiment in which TNT-degrading microorganisms could be isolated for purposes of bioremediation
Also indicate what experimental evidence would be useful to isolate TNT-degrading microorganisms.
What will be an ideal response?
Answer: Experimental designs will vary, but one example would be to use the enrichment culture technique with soil from an ammunition site. While adding TNT to the enrichment culture, a key piece of experimental evidence could be the loss of TNT in the culture to initiate isolation attempts.
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