Briefly list the basic steps involved in constructing a DNA fingerprint for a paternity case

What will be an ideal response?


Cut the DNA of each sample (child, mother, and supposed father) with restriction enzymes; separate the fragments for each sample with electrophoresis; perform a Southern blot analysis to transfer the fragments to a filter; probe the fragments with radiolabeled DNA oligonucleotides; and develop an autoradiogram to read the fragment sizes of each individual.

Biology & Microbiology

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The coding sequence in the DNA of ________ is normally continuous; that is, it is not interrupted by noncoding sequences.

A. mammals B. most insects C. prokaryotes D. plants

Biology & Microbiology

Though legless, the caecilian worm, Dermophis mexicanus is still classified with the amphibians. To which order do the caecilian worms belong?

a. Urodela b. Anura c. Apoda d. Salientia

Biology & Microbiology

Which protein is defective in cases of Duchenne muscular dystrophy?

a. dystrophin b. albumin c. fibrillin d. fibrinogen e. fibrin

Biology & Microbiology

Spreading black necrosis, swelling, pain and froth or bubbles are characteristic of

A) anthrax. B) gas gangrene. C) necrotizing fasciitis. D) chromoblastomycosis. E) Pseudomonas infection.

Biology & Microbiology