You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism
You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment, but you don't know which one. How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?
What will be an ideal response?
Answer: Fusion proteins of the proteins of interest plus a fluorescent protein, such as green fluorescent protein, could be made and introduced into the organism. Because the unique sequence is at the C-terminus, it may work best to fuse the GFP to the N-terminus of the proteins of interest.
You might also like to view...
Eating undercooked meat can lead to food-borne disease via helminthic infection. What is the common host for Taenia solium?
A. Ducks B. Cows C. Chickens D. Geese E. Pigs
To obtain large, flat cookies, one should use more fat in a recipe
Indicate whether the statement is true or false
Inability of the pancreas to produce insulin will result in
Suppose you hypothesized that the average salary in the company is $75,000. You then collect a sample of salaries for employees of the company. Using statistical theory, you know that, if the average salary is $75,000, then the probability that your sample average is above $90,000 or below $60,000 is 0.05. If you observe an average salary of $91,000:
A. you make the deductive argument that the probability that your sample average is above $90,000 or below $60,000 is not 0.05. The strength of this argument is 95%. B. you make the inductive argument that the average salary in the company is not $75,000. The strength of this argument is 95%. C. you make the inductive argument that the probability that your sample average is above $90,000 or below $60,000 is not 0.05. The strength of this argument is 5%. D. None of the answers is correct.