You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism

You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment, but you don't know which one. How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?
What will be an ideal response?


Answer: Fusion proteins of the proteins of interest plus a fluorescent protein, such as green fluorescent protein, could be made and introduced into the organism. Because the unique sequence is at the C-terminus, it may work best to fuse the GFP to the N-terminus of the proteins of interest.

Biology & Microbiology

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Indicate whether the statement is true or false

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Suppose you hypothesized that the average salary in the company is $75,000. You then collect a sample of salaries for employees of the company. Using statistical theory, you know that, if the average salary is $75,000, then the probability that your sample average is above $90,000 or below $60,000 is 0.05. If you observe an average salary of $91,000:

A. you make the deductive argument that the probability that your sample average is above $90,000 or below $60,000 is not 0.05. The strength of this argument is 95%. B. you make the inductive argument that the average salary in the company is not $75,000. The strength of this argument is 95%. C. you make the inductive argument that the probability that your sample average is above $90,000 or below $60,000 is not 0.05. The strength of this argument is 5%. D. None of the answers is correct.

Biology & Microbiology