AMP-PNP is a non-hydrolyzable analog of ATP that can bind to proteins in a similar manner as ATP but is no longer hydrolyzed. Predict what would happen to helicase activity if AMP-PNP were added to a DNA replication reaction.
A. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.
B. Helicase would function normally, since AMP-PNP is not the same as ATP and would not bind to helicase.
C. Helicase would function faster than normal, since the AMP-PNP is not hydrolyzed. ATP binding induces conformational changes, but hydrolysis slows DNA unwinding by helicase.
D. Helicase would function normally, since AMP-PNP is an analog of ATP and would function in the place of ATP.
Ans: A. Helicase would no longer function, since the AMP-PNP is not hydrolyzed. ATP binding and hydrolysis induce the conformational changes that facilitate DNA unwinding by helicase.
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