Plasma from a sick mouse is filtered across a membrane that has a 200 nm pore size. If the filtrate is still infectious when injected into a healthy mouse, then the pathogen is most likely a(n):  

A.  bacterium
B.  archaean
C.  fungus
D.  virus
E.  parasitic worm

Clarify Question
· What is the key concept addressed by the question?
· What type of thinking is required?

  Gather Content
· What do you already know about virus size?
 
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?

  Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
 


D.  virus

Clarify Question
· What is the key concept addressed by the question?
        o This question addresses virus size.
· What type of thinking is required?
        o This question is asking you to take what you already know and apply it to this unfamiliar situation.

  Gather Content
· What do you already know about virus size?
        o A traditional definition of viruses was that they were “filterable agents.” That is, infectious agents that could pass through a 500 nm filter.
        o Some viruses, like Mimivirus or Pandoravirus, can be larger. However, anything smaller than 200 nm is likely to be a virus.
        o The smallest bacterium, Mycoplasma, is between 200-300 nm across.    
 
Choose Answer
· Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?
        o Viruses are the smallest of the options listed. Bacteria, archaea, a fungus or worm would all be caught in the filter.

  Reflect on Process
· Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
        o The question required you to take what you already know and apply it to this unfamiliar situation.
        o Did you recognize that viruses are smaller than bacteria, archaea, fungi, or worms?

Biology & Microbiology

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