For a science fair project, two students decided to repeat the Hershey and Chase experiment, with modifications. They decided to radioactively label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogen atoms. Thus, labeling the nitrogen atoms would provide a stronger signal than labeling the phosphates. Why won't this experiment work?
a) Although there are more nitrogens in a nucleotide, labeled phosphates actually have 16 extra neutrons; therefore, they are more radioactive
b) Radioactive nitrogen has a half-life of 100,000 years, and the material would be too dangerous for too long
c) Amino acids (and thus proteins) also have nitrogen atoms; thus, the radioactivity would not distinguish between DNA and proteins
d) There is no radioactive isotope of nitrogen
Ans: c) Amino acids (and thus proteins) also have nitrogen atoms; thus, the radioactivity would not distinguish between DNA and proteins
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