Optimal recovery and identification of anaerobic bacteria in clinical specimens occurs when:
a. the initial incubation period is at 35°C to 37°C for 48 hours.
b. plates are incubated for at least 3 days before discarding as negative.
c. the initial incubation period is at 35°C to 37°C for 24 hours.
d. thioglycollate broth can be inspected daily for 3 days.
A
Inoculated plates should be immediately incubated under anaerobic conditions at 35°C to 37°C for 48 hours. In general, cultures should not be exposed to oxygen until after 48 hours' incuba-tion; anaerobes are most sensitive to oxygen during their long phase of growth. In addition, co-lonial morphologic characteristics often dramatically change between 24 and 48 hours.
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