Outline a procedure for creating a "knockout" microbe.

What will be an ideal response?


Many variations are possible. The answer should include (1) what organism is used as the source of DNA and how the desired DNA sequence is obtained, (2) the approach to modifying the gene, (3) the method of introduction of the modification into cells, and (4) how recombinant cells are identified. Since the question states a goal of a recombinant product, an expression vector should be specified. A variety of tools and techniques will be involved in a full answer. An excellent answer will include use of the CRISPR-Cas9 system.

Biology & Microbiology

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A strap of dense connective tissue that connects muscle to bone is (a)

a. ligament. b. cartilage. c. myofibril. d. tendon. e. filament.

Biology & Microbiology

Which of the following DNA sequences would most likely be recognized by a restriction enzyme? (Note: Only the coding strand is shown.)

A: AGTCGA B: GTCTGA C: TAAGGT D: TCGTGGA E: TAGCTA

Biology & Microbiology

major causes of diarrhea

What will be an ideal response?

Biology & Microbiology

Choose the letter of the best match from the following:A. abscisic acidB. trichomesC. aquaporinD. endodermisE. stomaRegulates movement of mineral ions into root xylem.

Fill in the blank(s) with the appropriate word(s).

Biology & Microbiology