A microbiologist is working with a bacteria strain to determine if cells are competent. Cells are grown under optimal conditions with DNA fragments that carry two genes, green fluorescent protein and ampicillin resistance, and then plated to nutrient agar with and without ampicillin. Following incubation, plates are examined for fluorescent colonies under a UV lamp. Predict the results if cells are competent.
A. Colonies will be present on both types of plates, but will only fluoresce on the ampicillin agar plates.
B. Fluorescent colonies will be present on the the ampicillin agar plates, but there will be no growth on the nutrient agar plates.
C. Fluorescent colonies will be present on both types of plates.
D. There will be no growth on either type of plate.
Answer: C
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