You are performing an experiment to assay phosphorylation of a substrate by a protein kinase. Based on the data in the table below, what do these experimental results suggest? SubstrateProtein kinaseGTPPhosphorylation detectedTest sample+++yesControl #1-++noControl #2+-+yesControl #3++-no
A. GTP is not required for phosphorylation.
B. The substrate can undergo autophosphorylation.
C. The protein kinase is required for phosphorylation.
D. The substrate is not required for phosphorylation.
Answer: B
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