When developing a monoclonal antibody-based test kit, great care must be taken to choose an antibody with the right binding properties. Imagine that you are developing a food-testing kit to specifically detect E. coli 0157:H7, a pathogenic serotype of E. coli that causes food poisoning. Choose the hybridoma line best suited for use in your test kit.
A. 1
B. 2
C. 3
D. 4
E. 5
Clarify Question
What is the key concept addressed by the question?
What type of thinking is required?
Gather Content
What do you already know about hybridomas? What other information is related to the question?
Choose Answer
Given what you now know, what information is most likely to produce the correct answer?
Reflect on Process
Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
C. 3
Clarify Question
What is the key concept addressed by the question?
· This question is asking about different hybridoma lines and their use in detecting E.coli 0157:H7 What type of thinking is required?
· Evaluate level:
o This question is asking you to weight and judge, or evaluate, the best hybridoma line to identify a specific pathogen.
Gather Content
What do you already know about hybridomas? What other information is related to the question?
· A hybridoma is made from the fusion of a lymphocyte and a tumor cell.
· Hybridomas are used to produce antibodies.
· The best test would indicate the specific pathogen with no ambiguity.
Choose Answer
Given what you now know, what information is most likely to produce the correct answer?
· The only hybridoma line that identifies E coli 0:157:H7 specifically, without indicating any other bacterium, is hybridoma 3.
Reflect on Process
Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
· Evaluate level:
o Answering this question correctly depended on your ability to weight and judge, or evaluate, various hybridomas. If you got an incorrect answer, did you remember that hybrodoma 3 is the only hybridomas that specifically identifies E.coli 0157:H7 and nothing else? Did you have trouble weighing the merits of the different hybrodomas to determine the correct answer?
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