The serum bactericidal test (SBT) involves:
a. serial dilutions of antimicrobials in ca-tion-adjusted Mueller-Hinton broth.
b. 99.9% reduction in CFU/mL, compared with the organism concentration in the original inoculum.
c. using the patient's serum to detect its bacteriostatic and bactericidal activity.
d. subculturing the tubes of a completed broth dilution test showing no visible growth.
C
The SBT tests the patient's serum for antibiotic activity that reflects not only the antibiotic con-centration attained, but also other serum factors that impact the effect of the antibiotic in vivo. Although the test is used to evaluate whether effective bactericidal concentrations are being achieved, the predictive clinical value for staphylococcal endocarditis or other infections caused by other bacteria remains uncertain.
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