Which of the following describes the construction of a metagenomic library?
A. A sample of ocean water is collected, the DNA is extracted and fragmented, then inserted into plasmid vectors before sequencing using the Sanger chain termination method.
B. A sample of ocean water is collected and the organisms present are sequenced, which allows construction of a library containing the whole genome.
C. A sample of ocean water is collected, the microbes are cultured on saline agar, then their DNA is extracted and sequenced using the Sanger method before constructing a library.
D. A sample of ocean water is collected, the DNA is extracted and fragmented, then next-generation sequencing leads to the alignment of the 100-300 bp sequences to known microbial genomes.
Answer: D
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